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KMID : 0861020120270030089
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Korea Journal of Herbology
2012 Volume.27 No. 3 p.89 ~ p.94
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Effects of Salvia Miltriorrhiza Radix on Neuronal Apoptosis following Intracerebral Hemorrhage of Rats
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Lee Hung-Gi
Kim Youn-Sub
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Abstract
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Objects: The purpose of this study was to observe the effect of Salviae Miltriorrhiza Radix(SMR) water-extract
on intracerebral hemorrhage(ICH) and neuronal apoptosis in the injured areas.
Methods: ICH was induced by the stereotaxic intrastriatal injection of bacterial collagenase type IV in
Sprague-Dawley rats. The rats were givened oral SMR treatment once a day for three days after the ICH
treatment. TUNEL positive cells in the affected regions were performed by TUNEL assay, Bax and Bcl-2
positive cells by immunohistochemistry and the Bax expression by western blotting method.
Results: The results are as follow;
1. SMR significantly reduced the number of TUNEL positive cells in the peri-hematoma reigions of ICH-induced
rats.
2. SMR significantly reduced the number of Bax positive cells in the peri-hematoma regions of ICH-induced
rats.
3. SMR did not affect the number of Bcl-2 positive cells in the peri-hematoma regions of ICH-induced rats.
4. SMR significantly reduced the Bax expressions compared with ICH group in hemorrhagic hemisphere of
ICH-induced rats.
Conclusion: These results suggest that SMR is effective in reducing neuronal apoptosis.
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KEYWORD
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Salvia Miltriorrhiza Radix(SMR), Collagenase, TUNEL, Bax, Bcl-2
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